Table 1 Genotype of engineered strains

GB05-Gtra-αβγ

GB2005, Δdcm(G135A), recA(A482G), Δdam, pspG::(tra-P_Rha-redαβγ)

GB05-CP4-57tra-αβγ

GB2005, Δdcm(G135A), recA(A482G), Δdam, yfjM::(tra-P_Rha-redαβγ)

GB05-recETtra-αβγ

GB2005, Δdcm(G135A), recA(A482G), Δdam, recET::(tra-P_Rha-redαβγ)

GB06-Gtra-αβγ

GB2006, gyrA(A259G), Δdcm(G135A), recA(A482G), Δdam, pspG::(tra-P_Rha-redαβγ)

GB06-CP4-57tra-αβγ

GB2006, gyrA(A259G), Δdcm(G135A), recA(A482G), Δdam, yfjM::(tra-P_Rha-redαβγ)

GB06-DLP12tra-αβγ

GB2006, gyrA(A259G), Δdcm(G135A), recA(A482G), Δdam, ybcW::(tra-P_Rha-redαβγ)

GB06-recETtra-αβγ

GB2006, gyrA(A259G), Δdcm(G135A), recA(A482G), Δdam, recET::(tra-P_Rha-redαβγ)

DH5G-Gtra-αβγ

DH5G, gyrA(A259G), Δdcm(G135A), recA(A482G), Δdam, pspG::(tra-P_Rha-redαβγ)

DH5G-CP4-57tra-αβγ

DH5G, gyrA(A259G), Δdcm(G135A), recA(A482G), Δdam, yfjM::(tra-P_Rha-redαβγ)

DH5G-DLP12tra-αβγ

DH5G, gyrA(A259G), Δdcm(G135A), recA(A482G), Δdam, ybcW::(tra-P_Rha-redαβγ)

DH5G-recETtra-αβγ

DH5G, gyrA(A259G), Δdcm(G135A), recA(A482G), Δdam, recET::(tra-P_Rha-redαβγ)

S17-1Δmu

S17-1, Δdcm(G135A), recA(C233T), Δdam, ΔMu