Fig. 5
Enhancing supply of acetyl-CoA and increasing the copy number of sclareol synthase for further sclareol production (A) Schematic diagram for increasing acetyl-CoA supply. (B) Titers and OD600 of strains which separately overexpressed lipolytic pathway genes. (C) Titers and OD600 of strains which separately deleted the lipid synthesis pathway genes. (D) Effects of increasing SsSL copy numbers on the titers of sclareol and GGOH. (E) The OD600 curves of strain YAs22-24 during 6-days-long fermentation. (F) Glucose consumption curves of strain YAs22-24 during 6-days-long fermentation. Bars and error bars represent the mean and standard deviation of three biological replicates. *p < 0.05, **p < 0.01, ****p < 0.0001