Skip to main content
Fig. 3 | Microbial Cell Factories

Fig. 3

From: Combinatorial metabolic engineering of Yarrowia lipolytica for high-level production of the plant-derived diterpenoid sclareol

Fig. 3

Enhancement of sclareol synthesis via screening of GGPP synthase and promoters. (A) Schematic diagram of pINA1269 plasmid expressing GGPPS from different sources. (B) Screening for GGPPS genes to increase GGPP content. (C) The SL genes initiated by pFBAin, pGPD, pTEFin and pTEFxo at the D069 site. (D) Comparison of sclareol production in different promoters express SL. (E) The cell density (OD600) curves of strain YAs3-7 during 6-days-long fermentation. (F) Glucose consumption curves of strain YAs3-7 during 6-days-long fermentation. Bars and error bars represent the mean and standard deviation of three biological replicates

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us