Fig. 5

Addition of tryptophan derivatives to the biosynthetic platform allows incorporation of substituted tryptophans into CM/NCM. (A) LC-MS analysis of metabolites of 200056 when fed 5/6/7-F/Cl/Br-Trp. Corresponding extracted ion chromatogram (EIC) was shown on the top. (B) Tryptophan derivatives tested. Dark blue shows tryptophan derivatives that were incorporated into an analogue of NCM (> 38% of underivatized CM by LC-MS analysis; Table S3) and further purified; medium blue indicates tryptophan derivatives that were incorporated into an analogue of NCM at lower levels (1–12% of underivatized CM by LC-MS analysis; Table S3) but were not further verified by purification; and light blue indicates tryptophan derivatives that did not show detectable incorporation into NCM