Fig. 2

Heterologous production of CM and NCM in Streptomyces. (A) Schematic representation of constructions and heterologous expressions of pL-CxnA₁-F and pL-CxnB-F. (B) Production of NCM in different Streptomyces hosts which possessed pL-CxnB-F when fermented on ISP2 plate. The yield of NCM in each strain was compared with that in the wild-type strain 200056 (Student’s t test, ^*p < 0.05, ^***p < 0.001). (C) Production of CM and NCM in different Streptomyces hosts which possessed pL-CxnA₁-F when fermented on ISP2 plate. The yields of CM and NCM in each strain were compared with those in the wild-type strain 200056 (Student’s t test or Welch’s t test, ^*p < 0.05, ^**p < 0.01). (D) Optimization of VB₁₂ concentration fed to fermentation medium ISP2 in S. coelicolor M1452/pL-CxnA₁-F. (E) Production of CM and NCM in Streptomyces/pL-CxnA₁-F with 100 µg/ml VB₁₂ fed to ISP2 plate. The yields of CM and NCM in each strain were compared with those in the wild-type strain 200056 (Student’s t test or Welch’s t test, ^*p < 0.05, ^***p < 0.001). Values are presented as mean ± SEM (three independent conjugants for each strain)