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Table 1 Factors and levels of independent variables

From: Scale up of fermentation of recombinant Escherichia coli for efficient production of spider drag silk protein MaSp1s and its dimers

Independent variables

Symbols

Range and levels

−1

0

+ 1

Induce temperature (°C)

A

20

25

30

Induce duration(h)

B

12

24

36

The concentration of IPTG (%)

C

0.2

0.4

0.6