From: Scale up of fermentation of recombinant Escherichia coli for efficient production of spider drag silk protein MaSp1s and its dimers
Independent variables
Symbols
Range and levels
−1
0
+ 1
Induce temperature (°C)
A
20
25
30
Induce duration(h)
B
12
24
36
The concentration of IPTG (%)
C
0.2
0.4
0.6