The knowledge driven DBTL cycle provides mechanistic insights while optimising dopamine production in Escherichia coli

Table 3 Summary of the key characteristics of different l-tyrosine production strains including the strains constructed in this study

Strain	Genotype	Rel. transcription tyrA	l-Tyrosine [g/L]	Resource
E. coli K12 ΔtyrR	K12 ΔtyrR pCL1920::_PLtetO−1aroG^fbrtyrA^fbrppsAtktA^b	–	0.62 ± 0.026	[67]
F	E. coli MG1655 pBbA5a::tyrB-tyrA-aroC T1-P_trc-aroA-aroL pBbB5c::aroE-aroD-aroBop-aroG-ppsA-tktA	–	2.17 ± 0.38	[68]
SCK5	W3110 ΔtyrR aroG:: P_{BBa_J23100}-synUTR_aroG-aroG^fbr tyrA:: P_{BBa_J23100}-synUTR_tyrA-tyrA^fbr P_{aroABCDELtyrB}-UTR_{aroABCDELtyrB}:: P_{BBa_J23100}-synUTR_{aroABCDELtyrB} P_ppsA-UTR_ppsA:: P_{BBa_J23100}-synUTR_ppsA(V4)	–	0.52 ± 0.17	[69]
FUS4	LJ110 ∆(pheA tyrA aroF) Δlac::P_tac::aroFBL⁺	0.05 ± 0.01	–	[63]
FUS4.T1	LJ110 Δ(∆(pheA tyrA aroF) Δlac::P_tac::aroFBL⁺ Δxyl::P_tac::tyrA (Y263C)	30.59 ± 1.20	0.60 ± 0.02	This study
FUS4.T2	LJ110 ∆(pheA tyrA aroF) Δlac::P_tac::aroFBL⁺ Δxyl::P_tac::tyrA (Y263C) ∆tyrR	48.01 ± 1.32	0.83 ± 0.01	This study

The strains E. coli FUS4, E. coli FUS4.T1, and E. coli FUS4.T2 were investigated in relative transcription of tyrA (n = 3) and l-tyrosine production. Relative expression was calculated according to the housekeeping gene ftsZ. l-tyrosine concentration was measured after 52 h of cultivation in a microbioreactor device (n = 3)

ISSN: 1475-2859