Table 2 Plasmids used in this study

pET 20b + 

P_lac; Amp^R; lacI^q

Merck (Darmstadt)

pET_hpaBC

P_lac; Amp^R; lacI^q

This study

pET_ddc

P_lac; Amp^R; lacI^q

This study

pJNTN-m-L

P_tac; Kan^R; lacI^q

[64]

pJNTN_hpaBC

P_tac; Kan^R; lacI^q

This study

pJNTN_ddc

P_tac; Kan^R; lacI^q

This study

pJNTN_hpaBC_ddc

P_tac; Kan^R; lacI^q

This study

pTarget-sgRNA-xylAB

Cm^R, ColE1ori

This study

pTarget-sgRNA-tyrR

Cm^R, ColE1ori

This study

pCas

Rep101^Ts, Km^R

[65]

pTarget-F

Str^R, ColE1ori

[65]

pTarget-cat

Cm^R gene, ColE1ori

This study

pJF119-tyrA^Tyr263Cys

Amp^R, ColE1ori

Unpublished