Table 1 Strains used in this study

E. coli DH5α

supE44 ΔlacU169 (Φ80lacZ_M15) hsdR17 recA1gyrA96 thi-1 relA1

[62]

E. coli FUS4

Wild type W3110 (F⁻, λ⁻ IN (rrnD-rrnE) 1, rph-1) ∆(pheA tyrA aroF) Δlac::P_tac::aroFBL⁺

[63]

E. coli FUS4.T1

Wild type W3110 (F⁻, λ⁻ IN (rrnD-rrnE) 1, rph-1) ∆(pheA tyrA aroF) Δlac::P_tac::aroFBL⁺ Δxyl::P_tac::tyrA (Y263C)

(This study)

E. coli FUS4.T2

Wild type W3110 (F⁻, λ⁻ IN (rrnD-rrnE) 1, rph-1) ∆(pheA tyrA aroF) Δlac::P_tac::aroFBL⁺ Δxyl::P_tac::tyrA (Y263C) ∆tyrR

(This study)