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Fig. 3 | Microbial Cell Factories

Fig. 3

From: The knowledge driven DBTL cycle provides mechanistic insights while optimising dopamine production in Escherichia coli

Fig. 3

In vitro and in vivo investigation of dopamine production in E. coli FUS4.T2. A Supernatants of lysed cells were mixed in different ratios with increasing amounts of HpaBC. Data represent mean of replicates (n = 3) with standard deviation as error bars. B For in vivo investigation of different hpaBC:ddc ratio duplicates were made using different RBS sequences (black diamond) compared with the in vitro results (green square). HpaBC: 4-hydroxyphenylacetate 3-monooxygenase; Ddc: l-DOPA decarboxylase; TIR: translation initiation rate; RBS: ribosome binding site

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Microbial Cell Factories

ISSN: 1475-2859

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