Fig. 5
From: Chaperone overexpression boosts heterologous small molecule production in Saccharomyces cerevisiae
mRFP signal and aspulvinone E production in strains transformed with MelA C-terminally tagged with mRFP. (A) mRFP signal measured for a 100 mL SD-urea shake-flask culture over the course of 120 h. The background signal observed for CEN.PK113-7D was subtracted from the SSA1 + YDJ1 strain and the reference strain (APS008) values. (B) aspulvinone E production in SSA1 + YDJ1 and reference strain harvested at 120 h. Columns represent average aspulvinone E production, as measured by the UV absorption at 368 nm of the peak corresponding to aspulvinone E and normalized to the reference strain. The asterisk indicates that the difference in aspulvinone E production is significantly different from the reference strain (p < 0.05). Error bars represent ± SD, n = 3