Fig. 1
From: Chaperone overexpression boosts heterologous small molecule production in Saccharomyces cerevisiae
Mating based setup for identification of beneficial chaperone genes. (A) Left: construction of the chaperone strain library by integration of chaperone GECs in integration sites X-2 and X-4. Right: construction of the query strain by integration of an SM GEC in the integration site XII-5 and simultaneous replacement of the ura3-52 marker gene with a selectable kanMX cassette. (B) Left: query and chaperone strains are arrayed. Middle: the two strain types are combined on solid YPG medium for mating and transferred to solid SC-Ura + Gal + G418 to select for diploids by replica-pinning. Right: diploids are transferred to solid medium compatible with SM production and quantitative detection