Fig. 6

Combinatorial multiplex repression using dCas12a-mediated CRISPRi system for isobutanol (IB)/3-methyl-1-butanol (3M1B) production in Synechocystis. See Table 1 for the details of the strains. Three millimolar rhamnose were added on day 0 for induction in individual strain. A IB and 3M1B production per cell of the engineered Synechocystis strains HX11-EVC, HX11-gltA, HX11-ppc, and HX106 on day 3. B IB and 3M1B production per cell of the engineered Synechocystis strains HX106, HX11-acnB, and HX107 on day 3. C IB and 3M1B production per cell of the engineered Synechocystis strains HX106, HX11-ppsA, and HX108 on day 3. D IB and 3M1B production per cell of the engineered Synechocystis strains HX106, HX11-cpcB, and HX109 on day 3. E IB and 3M1B production per cell of the engineered Synechocystis strains HX106, HX11-accC, and HX110 on day 3. F IB and 3M1B production per cell of the engineered Synechocystis strains HX11-ppc, HX11-ppsA, HX11-ccmA, and HX111 on day 3. G IB and 3M1B production per cell of the engineered Synechocystis strains HX11-ppsA, HX11-accC, HX11-pdh, and HX112 on day 3. H Summary of the strains and their respective targets included in this experiment for repression with the CRISPRi system. Results are the mean of three biological replicates. Error bars represent standard deviation. Asterisk represents significant difference between recombinant strains and the control strain (t-Test, *p < 0.05, **p < 0.005)