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Fig. 3 | Microbial Cell Factories

Fig. 3

From: dCas12a-mediated CRISPR interference for multiplex gene repression in cyanobacteria for enhanced isobutanol and 3-methyl-1-butanol production

Fig. 3

Establishment of dCas12a-mediated CRISPRi system in isobutanol (IB)/3-methyl-1-butanol (3M1B)-producing Synechocystis strain HX11. A Relative mRNA expression level of ppc, cpcB, and ppsA genes in HX11-ppc, HX11-cpcB, and HX11-ppsA strains, respectively, compared to the level of HX11-EVC strain. B IB production per cell of each strain with selected gene repressed on Day 5. C 3M1B production per cell of each strain with selected gene repressed on Day 5. For IB/3M1B production, results are the mean of two biological replicates; for mRNA relative expression level, results are the mean of three technical replicates. Error bars represent standard deviation. Asterisk represents significant difference between different strains (t-Test, *p < 0.05, **p < 0.005)

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Microbial Cell Factories

ISSN: 1475-2859

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