Fig. 5
Design of the challenge experiment. Medaka fish larvae were splitted into 7 groups (n > 30). Larvae of five groups were treated (immersion for 2 h) with either spores, free Hsp33, free OmpK21/260, spore adsorbed Hsp33 or spore adsorbed OmpK21/260. After 48 h of incubation in fresh media, the five treated groups and one of the two not treated were challenged with the pathogen and the survival rate monitored for additional 48 h