Fig. 3
Flow cytometry analysis of MV30 spores adsorbed with Hsp33 or OmpK21/260. 50.000 spores were analyzed for each sample. The percentage of MV30 spores that adsorbed Hsp33 or OmpK 21/260 is indicated. Red lines indicate the fluorescence signals of MV30 spores adsorbed with Hsp33 (A) or OmpK21/260(B) and reacted with anti-6xHis primary antibody and fluorescently conjugated secondary antibody. Black curves indicate the background fluorescence signals of MV30 spores not adsorbed with any of the antigens. The red line represents the threshold level based on the negative control