Fig. 2

Adsorption of Hsp33 or OmpK21/260 on MV30 spores. Western Blot analysis of MV30 spore surface proteins extracted after adsorption with 15 µg (lanes 1) or 20 µg (lanes 2) of Hsp33 (A) or OmpK21/260(B). Dot blot analysis performed with the serial dilutions of the supernatant fraction of the adsorption reaction with 15 µg (lanes 1) or 20 µg (lanes 2) of Hsp33 (C) or OmpK21/260(D). ST = pure Hsp33 (C) or OmpK21/260 (D). Immune reactions were performed using anti-6xHis primary antibody and peroxidase conjugated secondary antibody