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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Probiotic spore-based antigen delivery: a novel oral vaccine strategy against Vibrio infections in aquaculture

Fig. 1

Efficiency of mRFP adsorption by spores of four probiotic strains. (A) Fluorescence microscopy analysis of spores adsorbed with 2 µg of mRFP in sodium citrate buffer at pH 3.5. Phase Contrast (PC) and fluorescence (RFP) images of spores of PY79, MV4, MV11, MV24 and MV30 strains are shown. Exposure time 500 ms, Scale bar 1 μm. (B) Quantitative analysis of fluorescence of 80 spores for each strain by ImageJ software (Metods). The X- axis indicates the analyzed strains, the Y-axis shows the total corrected cellular fluorescent (TCCF) value. Dunnett’s multiple comparisons test was used for statistical significance, ****P < 0.0001

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Microbial Cell Factories

ISSN: 1475-2859

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