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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Enhancement of spinosad production in Saccharopolyspora spinosa by overexpression of the complete 74-kb spinosyn gene cluster

Fig. 3

Effects of overexpression of spn on sporulation and morphology. a Comparison of morphology on solid media after 7 days of cultivation. b Microscopic image of single bacterial colonies of Sa. spinosa and Sa. spinosa-spn after 7 days of cultivation. c Transcriptional analysis of genes related to sporulation and hyphal differentiation by quantitative real-time PCR. RNA samples of the genes bldD, ssgA, whiA and whiB were isolated from spores at 3 d, while RNA samples of the gene fstZ were isolated from mycelia at 48 h. **p < 0.01, ***p < 0.001 and ****p < 0.0001. d Sanning electron microscopy images of hyphae from Sa. spinosa and Sa. spinosa-spn after 60 h of cultivation

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Microbial Cell Factories

ISSN: 1475-2859

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