Fig. 4

(A) Transcriptomic analysis of differential gene expression of C. albicans. After the C. albicans was cultured with 2-phenylethanol, the genes expression of C. albicans were detected by RNA-seq. (B) GO enrichment analyses of differential gene expression. (C) After cultured with 2-phenylethanol, the expression level of genes related to oxidative stress response in C. albicans, including TRR1, SOD1 and CAT1 were assayed by RT-PCR. (D) The expression level of hypha-associated genes including ALS1, ALS3 and HWP1 in C. albicans after cultured with 2-phenylethanol. (E) ROS level, (F) SOD activity, and (G) CAT activity in C. albicans after cultured with 2-phenylethanol. (H) The impact on intracellular ROS levels by deleting and overexpressing CAT1 in C. albicans. This value represents the mean ± SD from three replicates. Statistical significance was defined based on the different p-values: *p < 0.05, **p < 0.01, and ***p < 0.001, ns: The comparison between the two sets of data was not statistically significant