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Table 2 Overall process parameters in microaerobic conditions on lactose and Whey

From: Improving sustainable isopropanol production in engineered Escherichia coli W via oxygen limitation

 

Lactose

Whey

Phase

Aerobic

O-limited

Aerobic

O-limited

YIPA/LAC

[Cmol Cmol− 1]

0.159 ± 0.015

0.256 ± 0.003

0.267 ± 0.004

0.309 ± 0.024

YIPA/LAC

[mol mol− 1]

0.635 ± 0.061

1.02 ± 0.01

1.07 ± 0.01

1.23 ± 0.10

YACO/LAC

[Cmol Cmol− 1]

0.035 ± 0.004

0.042 ± 0.016

0.070 ± 0.006

0.050 ± 0.004

YACO/LAC

[mol mol− 1]

0.140 ± 0.014

0.167 ± 0.064

0.278 ± 0.023

0.200 ± 0.015

qp, ipa

[mmol g− 1 h− 1]

0.836 ± 0.035

2.757 ± 0.175

1.001 ± 0.020

2.215 ± 0.009

qp, aco

[mmol g− 1 h− 1]

0.399 ± 0.075

0.394 ± 0.113

0.261 ± 0.013

0.361 ± 0.001

Carbon balance [%]

108.2 ± 4.4

94.2 ± 4.9

123.8 ± 1.6

115.8 ± 4.8

  1. Aerobic batch phase (dO2 » 1 %) and oxygen-limited (O-limited) pulsed batch scenario with a constant stirrer speed of 1,000 rpm and ingas oxygen concentration of 30 %