Fig. 4

Extracellular amylase activities and transcript levels produced by engineering the critical region (UP and spacer) of SPluxI−4310spoVG35. (A) Sequence information for SPluxI−4310spoVG35. (B) Engineering the UP and spacer regions of SPluxI−4310spoVG35. (C) Transcriptional levels of amyZ1 mediated by LuxI/R devices containing various SPluxI−4310spoVG35 critical region mutants. The activities of enzymes generated by the same type of expression strategy are represented by the same colour. The error bars represent the standard deviation