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Fig. 8 | Microbial Cell Factories

Fig. 8

From: Linking a polyketide synthase gene cluster to 6-pentyl-alpha-pyrone, a Trichoderma metabolite with diverse bioactivities

Fig. 8

Analysis of 6-pentyl-alpha-pyrone (6-PP) production in T. atroviride wild type and ∆pks1 mutants. High-performance thin-layer chromatography (HPTLC) analysis of extracts from T. atroviride WT and pks1 deletion mutants (∆pks1A, ∆pks1B, ∆pks1C). Control (PDA), WT, and ∆pks1 mutants were incubated for 96 h at 25 °C under reduced light conditions. Metabolites were extracted from the agar and separated by HPTLC. Two µg 6-PP were applied as reference (6-PP). Metabolite patterns were visualized at 254 nm. White arrows indicate the band corresponding to 6-PP in the WT extract and the reference

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Microbial Cell Factories

ISSN: 1475-2859

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