Fig. 3

Analysis of phylogenetic relationship of T. atroviride Pks1 The phylogenetic tree was generated by comparing amino acid sequences of PKSs associated with known compound classes from other fungi to Trichoderma Pks1 orthologues. Neighbor-joining algorithm of MEGA11 was used. Numbers indicate the bootstrap probability values of observing the branch topology shown. Scale bar indicates substitutions per site. Proteins are color-coded according to their association with biosynthetic pathways of specific compound classes: yellow for anthraquinone biosynthesis, green for macrolide biosynthesis, blue for flavonoid biosynthesis, and red for pyrone biosynthesis. The UniProt database was used to retrieve protein names. Pks1 of T. atroviride is highlighted in bold. Organism abbreviations correspond to specific fungal species, followed by the respective protein name: Aa (Alternaria alternata) – PksJ (Polyketide synthase PksJ); Ac (Aspergillus candidus) – CfoA (Chalcone synthase CfoA); Af (Aspergillus fumigatus) – Pyr2 (Non-reducing polyketide synthase Pyr2); Afl (Aspergillus flavus) – Pks27 (Non-reducing polyketide synthase Pks27). An (Aspergillus nidulans) – AptA (Non-reducing polyketide synthase AptA), MdpG (Atrochrysone carboxylic acid synthase); Ani (Aspergillus niger) – OtaA (Ornithine aminotransferase), AlbA (Non-reducing polyketide synthase AlbA), Wa (Conidial yellow pigment biosynthesis polyketide synthase); As (Alternaria solani) – Sol1 (Prosolanapyrone synthase); At (Aspergillus terreus) – CtvA (Highly reducing polyketide synthase CtvA); Ca (Calcarisporium arbuscula) – AurA (Highly reducing polyketide synthase AurA); Ff (Fusarium fujikuroi) – Bik1 (Bikaverin polyketide synthase Bik1); Fg (Fusarium graminearum) – Zea1 (Non-reducing polyketide synthase ZEA1), Pks12 (Non-reducing polyketide synthase PKS12); Ma (Metarhizium album) – Pks1 (Polyketide synthase 1); Sc (Stachybotrys chartarum) – Sat8 (Non-reducing polyketide synthase SAT8); Ta (Trichoderma atroviride) – Pks1; Tas (Trichoderma asperellum) – Pks1; Tg (Trichoderma gamsii) – Pks1; Tr (Trichoderma reesei) – Pks4 (Predicted protein, involved in green pigment biosynthesis [58]); Pe (Penicillium expansum) – PatK (6-Methylsalicyclic acid synthase); Pf (Pestalotiopsis fici) – FnsA (Naringenin synthase); Pn (Pseudocercospora fijiensis) – Pks8-1 (Non-reducing polyketide synthase PKS8-1); Pp (Penicillium polonicum) – VerA (Highly reducing polyketide synthase VerA).