From: Nutritional optimization for bioprocess production of staphyloxanthin from Staphylococcus aureus with response surface methodology: promising anticancer scaffold targeting EGFR inhibition
Factors
Variables
Unit
Production level
Minimum (-1)
Intermediate (0)
Maximum (+ 1)
X1
Temperature
-
30
37
44
X2
pH
5
7
9
X3
Mannitol
g %
0.5
1.5
2.5
X4
Peptone
X5
Incubation time
hr
24
48
72
X6
ZnCl2
mM
10
20