Fig. 7

Extraction and purification of STX pigment. (A) pigmented cell pellets; (B) cell pellets lost their color after exhaustion with methanol; (C) colored supernatant collected after centrifugation; (D) silica gel column chromatography showing golden-yellow band (arrowed); (E) collection of the colored fraction after elution (arrowed); (F) evaporation of the mobile phase and collecting the pigment powder