A toolkit for facilitating markerless integration of expression cassettes in Komagataella phaffii via CRISPR/Cas9

Table 3 Sequencing and read mapping quality metrics for clones subjected to WGS

Strain name	No of PE sequence reads	No of contigs (depth < 5×)	Predicted no of copies of eGFP	Read mapping to	Mapped reads	Mean read depth	No of positions with zero read depth
04576_37	3,354,227	345	1	Genome	99.08%	54×	34,499
				Plasmid	0.03%	30×	1804
04576_38	2,157,059	419	5	Genome	99.11%	39×	35,099
				Plasmid	0.15%	131×	0
04576_35	2,506,535	282	14	Genome	98.99%	44×	34,952
				Plasmid	0.49%	250×	0
PFK1_21	3,029,252	357	1	Genome	99.11%	48×	36,484
				Plasmid	0.03%	27×	2137
PFK1_66	3,928,371	316	2	Genome	99.16%	65×	34,634
				Plasmid	0.05%	70×	1649
PFK1_72	3,422,011	328	3	Genome	99.08%	57×	35,164
				Plasmid	0.11%	147×	0
ROX1_97	4,626,752	320	1	Genome	99.22%	79×	32,727
				Plasmid	0.03%	47×	1878
ROX1_87	902,371	337	2	Genome	98.92%	19×	69,733
				Plasmid	0.14%	60×	0
ROX1_96	1,879,340	296	8	Genome	99.09%	37×	32,654
				Plasmid	0.14%	229×	0

Read mapping was performed against both reference genomes and donor cassette plasmids. Also shown are predicted number of eGFP copies based on the WGS data. Further data on relative coverages of eGFP is shown in Supplementary Tables S2 to S7

ISSN: 1475-2859