Fig. 2

Efficiency of CRISPR/Cas9-mediated markerless eGFP donor cassette integrations targeting 04576, PFK1, and ROX1 loci. A Transformation efficiency shown as the total number of transformants (cfu µg⁻¹ donor cassette DNA). B Integration efficiency shown as the percentage of transformants with integrated eGFP, determined by measuring green fluorescence for single clones in an endpoint screening assay after 48 h of growth in 96-well plates. Transformation reactions were incubated for 1, 2, and 3 h prior to selection on G418 agar. Data shown are mean values for three independent experiments with, in total, n = 2234 tested clones, and error bars show standard errors of the means