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Table 1 Strains and related plasmids in this study

From: Engineering a PhrC-RapC-SinR quorum sensing molecular switch for dynamic fine-tuning of menaquinone-7 synthesis in Bacillus subtilis

Names

Characteristics

Source

Strains

B.subtilis 168

 

lab stock

BS01

BS168::ΔaroA

this study

BS02

BS168::ΔaroH

this study

BS03

BS168::ΔtrpE

this study

BS04

BS168::ΔpabAB

this study

BS05

BS168::ΔdhbB

this study

BS06

BS168::ΔalsS-D

this study

BX00

BS168:: Pgrac100-sinR

this study

BX01

BS168:: Pgrac100-sinR::PtapA-eGFP

this study

BX02

BS168:: Pgrac100-sinR::PepsA-eGFP

this study

BX03

BS168:: Pgrac100-sinR::PspoIIA-eGFP

this study

BX04

BS168:: Pgrac100-sinR::PslrR-eGFP

this study

BX05

BS168:: Pgrac100-sinR::PaprE-eGFP

this study

BX06

BS168:: Pgrac100-sinR::Pepr-eGFP

this study

BX07

BS168:: Pgrac100-sinR::PsacB-eGFP

this study

BX08

BS168:: Pgrac100-sinR::PamyE-eGFP

this study

BX09

BS168:: Pgrac100-sinR::PcodY-eGFP

this study

BG1

BS168:: Pgrac100-sinR::Pe1-eGFP

this study

BG2

BS168:: Pgrac100-sinR::Pe2-eGFP

this study

BG3

BS168:: Pgrac100-sinR::Pe3-eGFP

this study

BG4

BS168:: Pgrac100-sinR::Pe4-eGFP

this study

BG5

BS168:: Pgrac100-sinR::Pe5-eGFP

this study

BG6

BS168:: Pgrac100-sinR::Pe6-eGFP

this study

BG7

BS168:: Pgrac100-sinR::Pe7-eGFP

this study

BG8

BS168:: Pgrac100-sinR::Pe8-eGFP

this study

BG9

BS168:: Pgrac100-sinR::Pe9-eGFP

this study

BG10

BS168:: Pgrac100-sinR::Pe10-eGFP

this study

BG11

BS168:: Pgrac100-sinR::Pe11-eGFP

this study

BG12

BS168:: Pgrac100-sinR::Pe12-eGFP

this study

BG13

BS168:: Pgrac100-sinR::Pe13-eGFP

this study

BG14

BS168:: Pgrac100-sinR::Pe14-eGFP

this study

BG15

BS168:: Pgrac100-sinR::Pe15-eGFP

this study

BG16

BS168:: Pgrac100-sinR::Pe16-eGFP

this study

BG17

BS168:: Pgrac100-sinR::Pe17-eGFP

this study

BG18

BS168:: Pgrac100-sinR::Pe18-eGFP

this study

BG19

BS168:: Pgrac100-sinR::Pe19-eGFP

this study

BP00

BS168::sinR-eGFP

this study

BP01

BS168::sinR-eGFP::Pgrac100-rapC

this study

BP02

BS168::sinR-eGFP::Pgrac100-phrC

this study

BP03

BS168::Pe9-eGFP

this study

BP04

BS168::Pe9-eGFP::Phag-rapC

this study

BP05

BS168::Pe9-eGFP::Phag-rapC::Phag-phrC

this study

BP06

BS168::Phag-phrC::Phag-rapC

this study

BW1

BS168::Phag-rapC::Phag-phrC::Pe11-aroA

this study

BW2

BS168::Phag-rapC::Phag-phrC::Pe11-aroA::Pe9-aroH

this study

BW3

BS168::Phag-rapC::Phag-phrC::Pe11-aroA::Pe9-aroH::Pe9-trpE

this study

BW4

BS168::Phag-rapC::Phag-phrC::Pe11-aroA::Pe9-aroH::Pe9-trpE::Pe9-dhbB

this study

BW5

BS168::Phag-rapC::Phag-phrC::Pe11-aroA::Pe9-aroH::Pe9-trpE::Pe9-dhbB::Pe9-alsA-D

this study

BW6

BS168::Phag-rapC::Phag-phrC::Pe11-aroA::Pe9-aroH::Pe9-trpE::Pe9-dhbB::Pe13-alsA-D

this study

BW7

BS168::Phag-rapC::Phag-phrC::Pe11-aroA::Pe9-aroH::Pe9-trpE::Pe9-dhbB::Pe16-alsA-D

this study

BW8

BS168::Phag-rapC::Phag-phrC::Pe11-aroA::Pe9-aroH::Pe9-trpE::Pe9-dhbB: Pe17-alsA-D

this study

BL21-eGFP

Containing eGFP

lab stock

plasmids

P7C6

Pmd18-T, containing lox71-zeo-lox66 cassette

lab stock

pHT-XCR6

Amp, Cmr, containing promoter Pgrac100

lab stock

pHT-Cre

Amp, Km, E. coli − B. subtilis shuttle vector, containing cre under the control of Pspac

lab stock