Fig. 3

Verification of the SinR Target Promoter. (A) SinR structure, the red dashed box shows the N-terminal domain; (B) simulated structure of SinR bound DNA; (C) the process of SinR regulation after induction by IPTG: in the absence of IPTG induction, BX00 expressed a small amount of SinR, unable to regulate eGFP expression in BX01-BX09 (two plots of the upper layer); upon the addition of IPTG, a large amount of SinR was produced, which bent the DNA in the target promoter region and inhibited the expression of eGFP(two plots of the lower layer); (D) before and after the addition of the IPTG, relative fluorescence intensity change of BX01-BX09 strain; (E) relative fluorescence intensity change of strains BX01, BX02 and BX04 with IPTG concentration