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Table 2 Kinetic parameters of RsNpcA towards various nitrophenols

From: Biodegradation of p-nitrophenol by Rhodococcus sp. 21391 unveils a two-component p-nitrophenol monooxygenase with broad substrate specificity

Substrate

Km (μM)

kcat (min−1)

kcat/ Km (M−1 s−1)

 

PNP

56.70 ± 9.60

4.74 ± 0.60

1415.68 ± 56.07

This study

2NP

N.D

N.D

N.D

2C4NP

21.94 ± 3.34

1.62 ± 0.10

1231.79 ± 116.25

4FP

630.42 ± 118.90

1.98 ± 0.36

52.3 ± 0.30

4CP

31.41 ± 0.64

0.66 ± 0.01

343.72 ± 13.53

4BP

15.57 ± 0.83

0.60 ± 0.01

642.75 ± 28.25

4IP

208.53 ± 39.33

0.294 ± 0.03

23.57 ± 1.92

PNP

25.4 ± 3.63

–

–

Ref. [30]

PNP

20.3 ± 2.70

–

11,083.33 ± 716.66

Ref. [31]

  1. Enzyme assay was carried out in 50 mM Tris–HCl buffer (pH 7.5) at 25 °C, with 800 μM NADH and 20 μM FAD
  2. PNP p-nitrophenol, 2NP o-nitrophenol, 2C4NP 2-chloro-4-nitrophenol, 4FP p-fluorophenol, 4CP p-chlorophenol, 4-BP p-bromophenol, 4IP p-iodophenol, N.D. no activity detected
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Microbial Cell Factories

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