Optimization of thermostable amylolytic enzyme production from Bacillus cereus isolated from a recreational warm spring via Box Behnken design and response surface methodology

Table 5 Significance level and ANOVA for amylase production process

Source	Sum of squares	df	Mean square	F-value	p-value
Model	78,099.67	9	8677.74	181.86	< 0.0001
A-pH	54.36	1	54.36	1.14	0.3212
B-incubation time	580.61	1	580.61	12.17	0.0102
C-substrate concentration	6000.55	1	6000.55	125.75	< 0.0001
AB	339.83	1	339.83	7.12	0.0321
AC	2313.31	1	2313.31	48.48	0.0002
BC	909.65	1	909.65	19.06	0.0033
A²	52,298.85	1	52,298.85	1096.00	< 0.0001
B²	10,054.08	1	10,054.08	210.70	< 0.0001
C²	1290.47	1	1290.47	27.04	0.0013
Residual	334.02	7	47.72
Lack of Fit	264.98	3	88.33	5.12	0.0744
Pure Error	69.05	4	17.26
Cor Total	78,433.70	16

ISSN: 1475-2859