Table 1 Strains and genetic constructs used in this study
Strain | Construct | Description | References |
|---|---|---|---|
Syn-EVC | pEEK2-KmR | Synechocystis sp. PCC 6803 strain harboring pEEK2 plasmid for Km resistance as empty vector control | Mustila et al. (2021) |
Syn-RnKICD | pEEK2-RnKICD | Synechocystis sp. PCC 6803 strain harboring pEEK2 plasmid for Km resistance and RnKICD | Mustila et al. (2021) |
Syn-N363A | pEEK2-N363A | Synechocystis sp. PCC 6803 strain harboring pEEK2 plasmid for Km resistance and N363A mutation in RnKICD | This study |
Syn-F336V | pEEK2-F336V | Synechocystis sp. PCC 6803 strain harboring pEEK2 plasmid for Km resistance and F336V mutation in RnKICD | This study |
Syn-F336V/N363A | pEEK2-F336V/N363A | Synechocystis sp. PCC 6803 strain harboring pEEK2 plasmid for Km resistance and F336V/N363A mutation in RnKICD | This study |
EcBL21-EVC | pETBB-KmR | E. coli BL21(DE3) strain harboring pETBB plasmid for Km resistance as empty vector control | Mustila et al. (2021) |
EcBL21-RnKICD | pETBB-RnKICD | E. coli BL21(DE3) strain harboring pETBB plasmid for Km resistance and RnKICD | Mustila et al. (2021) |
EcBL21-Q251E | pETBB- Q251E | E. coli BL21(DE3) strain harboring pETBB plasmid for Km resistance and Q251E mutation in RnKICD | This study |
EcBL21- Q265E | pETBB- Q265E | E. coli BL21(DE3) strain harboring pETBB plasmid for Km resistance and Q265E mutation in RnKICD | This study |
EcBL21-N363A | pETBB-N363A | E. coli BL21(DE3) strain harboring pETBB plasmid for Km resistance and N363A mutation in RnKICD | This study |
EcBL21-F336V | pETBB-F336V | E. coli BL21(DE3) strain harboring pETBB plasmid for Km resistance and F336V mutation in RnKICD | This study |
EcBL21-F336V/N363A | pETBB-F336V/N363A | E. coli BL21(DE3) strain harboring pETBB plasmid for Km resistance and F336V/N363A mutation in RnKICD | This study |