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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Improved methyl supply for 5-methyltetrahydrofolate production in E. coli through a novel C1 transfer pathway

Fig. 3

LC-MS/MS analysis of 5-MTHF and isotope tracing of methyl transfer process. (A) Comparison and (B) quantitative comparison of 5-MTHF synthesis by cell lysates in vitro before and after the introduction of the exogenous methyl supply pathway. Tandem mass spectrometry fragmentation profile of (C) acetyl-CoA and (D) 5-MTHF in M1017A cell lysates incubated with unlabeled acetate (above) and D-labeled acetate (below) in positive ion mode. ND, not detected. The red numbers represent the precursor and fragment ions that differ in mass spectrum

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Microbial Cell Factories

ISSN: 1475-2859

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