Table 3 Box-Behnken (BB) design for optimizing the most effective variables on protease production
Trial | A:Peptone | B:Yeast extract | C:Sesame cake | Protease activity | Predicted values |
|---|---|---|---|---|---|
% | % | % | U/ml | U/ml | |
1 | (0) 1.5 | (0) 1.5 | (0) 0.75 | 365.5 | 367.70 |
2 | (0) 1.5 | (−) 1 | (−) 0.5 | 344.4 | 354.28 |
3 | (−) 1 | (−) 1 | (0) 0.75 | 317.8 | 301.01 |
4 | (+) 2 | (0) 1.5 | (+) 1 | 574 | 567.09 |
5 | (+) 2 | (−) 1 | (0) 0.75 | 441.5 | 435.41 |
6 | (0) 1.5 | (0) 1.5 | (0) 0.75 | 362.3 | 367.70 |
7 | (+) 2 | (+) 2 | (0) 0.75 | 630 | 646.79 |
8 | (0) 1.5 | (+) 2 | (+) 1 | 462.3 | 452.43 |
9 | (−) 1 | (0) 1.5 | (−) 0.5 | 414.1 | 421.01 |
10 | (0) 1.5 | (0) 1.5 | (0) 0.75 | 375.3 | 367.70 |
11 | (0) 1.5 | (−) 1 | (+) 1 | 470 | 483.00 |
12 | (−) 1 | (+) 2 | (0) 0.75 | 381.4 | 387.49 |
13 | (0) 1.5 | (+) 2 | (−) 0.5 | 695.7 | 682.70 |
14 | (+) 2 | (0) 1.5 | (−) 0.5 | 732 | 728.21 |
15 | (−) 1 | (0) 1.5 | (+) 1 | 476.8 | 480.59 |