Determining metabolic mechanism linking phospholipids and docosahexaenoic acid through phosphatidylcholine synthesis by phosphocholine cytidylyltransferase (CCT) overexpression in Schizochytrium sp

Cui, Xiaowen; Chen, Tingting; Meng, Yizhen; Pan, Xueshan; Wu, Ruizhe; Lu, Yinghua; Yao, Chuanyi; Lin, Xihuang; Ling, Xueping

doi:10.1186/s12934-025-02703-2

Table 4 Phospholipid species in SR21 and SR21-CCT

From: Determining metabolic mechanism linking phospholipids and docosahexaenoic acid through phosphatidylcholine synthesis by phosphocholine cytidylyltransferase (CCT) overexpression in Schizochytrium sp

Type	Unsaturation	Acyl composition	Content(µg/g)
Type	Unsaturation	Acyl composition	SR21-72 h	SR21-CCT − 72 h	SR21-120 h	SR21-CCT − 120 h
PC	C30:0	C14:0/C16:0	217.4 ± 3	363.1 ± 54.8^**	183.7 ± 73.6	287.7 ± 56.1
	C32:0	C16:0/C16:0	228.8 ± 40.9	1571 ± 255^**	215.6 ± 27.9	446 ± 9^**
	C36:5	C14:0/C22:5	0	144 ± 43.6^**	98.3 ± 38.8	187 ± 20.8^*
	C37:5	C15:0/C22:5	1046 ± 204.4	1883 ± 692.8	985.2 ± 207.3	1250 ± 97.2
	C37:6	C15:0/C22:6	6673.7 ± 527.6	6575.3 ± 846.7	5240.1 ± 1108.8	7520.8 ± 217.5^**
	C38:5	C16:0/C22:5	4574.4 ± 1247.7	8055.4 ± 1624.7^**	2348.7 ± 786.6	3826.3 ± 1266.6
	C38:6	C16:0/C22:6	10533.1 ± 2668.5	16262.3 ± 3120.7^*	5168.9 ± 1472.4	8466.7 ± 721^*
	C40:6	C18:0/C22:6	19.4 ± 4.9	244.9 ± 87.6^**	42.5 ± 13.9	349.9 ± 14.1^**
	C40:7	C18:1/C22:6	9 ± 0.2	146.9 ± 45.7^**	103.2 ± 40.9	262.7 ± 14.1^**
	C42:10	C20:4/C22:6	507.2 ± 154.2	427.1 ± 60.7	197.9 ± 27.3	901.2 ± 77.7^**
	C42:11	C20:5/C22:6	577 ± 113.5	873.4 ± 437.5	598.1 ± 235.6	1159.6 ± 187.4
	C44:10	C22:5/C22:5	1588.4 ± 23.6	2733.2 ± 1025.3	1295.4 ± 521	1697.7 ± 386.4
	C44:11	C22:5/C22:6	10955.8 ± 428.2	15857.4 ± 2690.5	7314 ± 2443.5	9346.4 ± 675.2
	C44:12	C22:6/C22:6	18541.1 ± 3154.3	31683.8 ± 2317.8	14552.4 ± 4889.3	18433.2 ± 1706.4
LPC	C15:0	C15:0	41.6 ± 0	1650.3 ± 155	493.4 ± 264.4	1257 ± 163.4^*
	C16:0	C16:0	113.4 ± 9	1458.6 ± 62.4^**	83.6 ± 15.4	74.7 ± 14.6
	C22:5	C22:5	3355.4 ± 334.7	4497.8 ± 1297.7	2215.5 ± 799.4	4622.1 ± 591.8^*
	C22:6	C22:6	8244.2 ± 1823.6	9657.7 ± 1134.4	4802.7 ± 2758.8	11424.7 ± 1112.7^*
PG	C31:0	C15:0/C16:0	153.8 ± 30.1	148.1 ± 72.1	105 ± 43.7	257.9 ± 61.7^*
	C32:0	C16:0/C16:0	13704.4 ± 1625	10050.9 ± 4131.6	3741.9 ± 1693	3204.4 ± 402.1
	C38:5	C16:0/C22:5	227.3 ± 53.8	381.3 ± 303.7	180.8 ± 87.3	115.1 ± 20.7
	C38:6	C16:0/C22:6	498.7 ± 48	283.9 ± 92.6	196.3 ± 90.1	361.3 ± 53.2^*
	C44:11	C22:5/C22:6	244 ± 92.1	197 ± 45.4	190.7 ± 120	415.3 ± 84.5^*
	C44:12	C22:6/C22:6	1319.1 ± 157.9	520.4 ± 106	492 ± 302.6	1071.7 ± 202.4^*
PI	C38:5	C16:0/C22:5	10830.1 ± 1331.7	24204.1 ± 3615.5	11439.8 ± 5890.9	8186.9 ± 704.7
	C38:6	C16:0/C22:6	13473.5 ± 805.2	48872.2 ± 772.7	6895 ± 1403.7	15964.3 ± 147.7^**
PE	C38:5	C16:0/C22:5	1175.1 ± 178.6	3217.4 ± 537.8^**	1014.3 ± 499.8	1251 ± 270.4
	C38:6	C16:0/C22:6	2702.9 ± 481	6396.3 ± 1541.7^**	2223.6 ± 899.3	2737 ± 456.7
	C44:11	C22:5/C22:6	1388.1 ± 384.2	1463.9 ± 215	563.1 ± 257.5	1412.9 ± 501.5^*
	C44:12	C22:6/C22:6	1073.9 ± 345.2	3864.6 ± 330.3^**	1314.1 ± 731.5	3547.3 ± 799.3^*
PS	C28:0	C14:0/C14:0	63.9 ± 24.7	52.2 ± 38	27 ± 7.8	26.8 ± 6.6
	C44:12	C22:6/C22:6	266.5 ± 24.3	86.8 ± 7.9^**	14.2 ± 4.8	56.5 ± 6.5^**
PA	C44:12	C22:6/C22:6	59.9 ± 3.7	250.1 ± 70.2^**	63.9 ± 6.1	81.8 ± 12.4^*

Note: *p-value < 0.05 and **p-value < 0.01, compared to SR21 at each time

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