Fig. 1

Evaluation of Aga2- and Sed1-based display systems in Saccharomyces cerevisiae (Sc) and Saccharomyces boulardii (Sb). (a) Schematic representation of the display cassettes expressed by plasmids. Each construct includes a signal peptide (Aga2sp, MFαsp, or Sed1sp), a G4S linker, yeGFP as a reporter protein, a myc tag for detection, and an anchor protein (Aga2 anchor or Sed1 anchor). Expression is driven by the GPD promoter with termination regulated by the CYC1 terminator. (b) Flow cytometry analysis of Sb expressing Aga2- or Sed1-based plasmids. Mean fluorescence intensity (MFI) values for yeGFP (green) and Alexa 594 (red) were analyzed separately. Each analysis was conducted with 10,000 cells. Error bars represent standard deviation (SD), and statistical significance was determined using one-way ANOVA (p < 0.05). Different lowercase letters denote significant differences, with green letters corresponding to yeGFP MFI and red letters corresponding to Alexa 594 MFI