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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Introduction of human m6Am methyltransferase PCIF1 facilitates the biosynthesis of terpenoids in Saccharomyces cerevisiae

Fig. 6

PCIF1 promoted longifolene production in engineered yeast. (A) The optimization of longifolene biosynthesis pathway. Erg10, acetoacetyl-CoA thiolase; Erg13, HMG-CoA synthase; EfMvaE, HMG-CoA reductase from E. faecalis; Erg12, mevalonate kinase; Erg8, phosphomevalonate kinase; Erg19, mevalonate pyrophosphate decarboxylase; Idi1, isopentenyl diphosphate: dimethylallyl diphosphate isomerase; Erg20, farnesyl pyrophosphate synthetase; ERG9, squalene synthase; PsLS, codon-optimized longifolene synthase. (B) GC-MS detection of longifolene standard and its production in engineered yeast. (C) The longifolene production in different yeast strains. The asterisks indicate significant differences (**p < 0.01, *p < 0.05)

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Microbial Cell Factories

ISSN: 1475-2859

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