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Table 1 Bacterial (E. coli) and yeast (Y. lipolytica) strains used in this study

From: Engineered yeast Yarrowia lipolytica as a chassis for biosynthesis of fatty acids from mannitol and macroalgal biomass extracts

Strain

Genotype or Plasmid

Source

E. coli

 DH5α

F − endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK-mK+), λ–

[19]

 DH5α

pUC-URA

[39]

 DH5α

pUC-URA-EYD1g

[41]

 DH5α

pAD_UT-DGA1g

[12]

Y. lipolytica

 AJD

MATA, A101: ura3-302

[29]

 AJD ΔEYD1

MATA, A101: ura3-302 ΔYALI0F01650g

[41]

 AJD ΔEYD1 ura-

MATA, A101 ΔEYD1

[41]

 AJD ΔEYD DGA1

MATA, A101: ura3-302 ΔEYD, overexpression of YALI0E32769g

This study

 AJD DGA1

MATA, A101: ura3-302, overexpression of YALI0E32769g

[12]