Fig. 5
From: A rapid and efficient strategy for combinatorial repression of multiple genes in Escherichia coli
Characterization of the multi-gene combinatorial repression system using E. coli MG1655RFP. Three sgRNA sites respectively targeting gusA, rfp, and lacZ were constructed into the p3gRNA-LTA plasmid. The sgRNA targeting gusA is expressed by the PlacO1 promoter. The sgRNA targeting rfp is expressed by the PLtetO−1 promoter. The sgRNA targeting lacZ is expressed by the ParaBAD promoter. “-” indicates that no inducer is added, and “+” indicates that the inducer is added. Strains without sgRNA targeting any genes were used as controls. The enzyme activity and fluorescence of the control were normalized to 1. Data are expressed as means (± s.d.) from three independent experiments