Fig. 1

Enhancing the natamycin production by protoplast fusion combined with KH2PO4 tolerance screening. (a) Flow diagrams of ARTP mutagenesis. (b) Flow diagrams of DES mutagenesis. (c) Flow diagrams of protoplast fusion. (d) and (e) Results of three rounds of ARTP mutagenesis. (f) and (g) Results of three rounds of DES mutagenesis. (h) and (i) Results of protoplast fusion. (j) and (k) Differences in natamycin production and mycelial growth between S. gilvosporeus ATCC13326 and S. gilvosporeus GR-2. DCW, cell dry weight. The error bars represent the standard error of the mean