Enhancing D-lactic acid production from methane through metabolic engineering of Methylomonas sp. DH-1

Table 2 Plasmids used in this study

Plasmid	Description	References
pDel2-glgA-Lm.LDH	pCM184-U_glgA-[Lm.LDH-T_rrnB-Kan^R]-D_glgA, without Amp^R	[25]
pDel3-glgA-Lm.LDH	pDel2-glgA-Lm.LDH, containing MauBI/BamHI site for promoter cloning	This study
pDel3-glgA-P_tet-Lm.LDH	U_glgA-[P_tetA-tetR-T_rrnB-P_tet-Lm.LDH-T_rrnB-Kan^R]-D_glgA	This study
pDel3-glgA-P_tac-Lm.LDH	U_glgA-[P_lacI-lacI-T_rrnB-P_tac-Lm.LDH-T_rrnB-Kan^R]-D_glgA	This study
pDel2-filE	Plasmid containing [U_fliE-T_rrnB-Kan^R-D_fliE] cassette for fliE gene deletion	[25]
pDel2-glgA	pDel2-[U_glgA-T_rrnB-Kan^R-D_glgA]	This study
PDel2-glgABC	pDel2-[U_glgA-T_rrnB-Kan^R-D_glgC]	This study
pDel2-glgC	pDel2-[U_glgC-T_rrnB-Amp^R-D_glgC]	This study

ISSN: 1475-2859