Fig. 3
Effect of glgC deletion on cell growth and LA tolerance. A Growth comparison of indicated strains in 125 mL flasks (12.5 mL NMS medium) and 500 mL flasks (50 mL NMS medium) to examine scale-up effects. The initial inoculation OD600 was 0.1. B Metabolic pathway of glycogen synthesis from methane in Methylomonas sp. DH-1, highlighting the key intermediates: F6P, fructose-6-P; G6P, Glucose-6-p; G1P, Glucose-1-p. The carbon flux under carbon excess conditions is illustrated with arrows for the JHM801(ΔglgA) and JHM802 (ΔglgABC) strains, highlighting ADP-glucose accumulation resulting from glgA deletion. C Effect of glgC deletion on cell growth of JHM804 under different culture scales. The initial inoculation OD600 was 0.2 and the strains were grown in 125 mL flask containing 12.5 mL NMS medium or 500 mL flask containing 50 mL NMS medium. Each value represents the average ± standard deviation of two independent experiments. D Effect of IPTG addition (50 µM) on cell growth and D-LA production in JHM805 with methane supplied every 24h, with the initial inoculation OD600 was 0.1. Error bars indicate standard deviations of two independent experiments