Fig. 4
(A) Pyruvate and (B) parapyruvate formation in bioreactor cultivations of V. natriegens Δvnp12 ΔaceE (black, square), V. natriegens Δvnp12 ΔaceE ΔligK (blue, circle) and induced V. natriegens Δvnp12 ΔaceE (pEKEx2-ligK) (red, inverted triangle). Strains were grown in 500 mL minimal medium at 37 °C with 100 g glucose L-1, 2 g acetate L-1 and a feed of 0.24 gAc h-1. Data points with error bars are means and standard deviations of independent triplicates. Data points without error bars represent means of duplicates