Fig. 3

Growth of V. natriegens WT (A, black, square) and V. natriegens Δvnp12 ΔaceE (B, black, square) compared to respective ligK deletion strains (blue, circle), as well as both backgrounds with the overexpression plasmid pEKEx2-ligK uninduced (orange, triangle) and induced (red, inverted triangle). Cultivations were performed in baffled shaking flasks containing 50 mL minimal medium with 7.5 g glucose L^-1 (A and B) and 1 g acetate L^-1 (B) at 37 °C. (C) Growth of V. natriegens WT and V. natriegens ΔligK with 10 mM PCA as only carbon and energy source in baffled shaking flasks containing 50 mL minimal medium at 37 °C. Data shown are means and standard deviations of independent triplicates. Asterisk indicates statistical significance of p < 0.05