Fig. 2
Pyruvate production in batch fermentations with V. natriegens Δvnp12 ΔaceE. Plotted are the cell dry weight (CDW, grey, square), pyruvate (Pyr, blue, triangle), acetate (Ace, green, diamond) and glucose (Glc, orange, circle) concentrations over time. Fermentations were performed in 500 mL minimal medium at 37 °C with (A) 100 g glucose L-1 and 2 g acetate L-1 as initial substrates and (B) using an additional feed of 0.24 gAc h-1. (C) Carbon distribution after 10 h in C-mol of found compound per C-mol of overall substrate for both bioreactor setups. Data shown are means and standard deviations of independent triplicates