Fig. 3

Miltiradiene and abietatriene productions in engineered C. glutamicum (A) A fed-batch cultivation of engineered strains (C. glutamicum HL01 harboring pBE-DI pKC) was conducted, starting with 2% (w/v) initial glucose, followed by a single glucose supplementation (1%, w/v) during the cultivation (left panel). Optical densities at 600 nm (OD₆₀₀, black square) and glucose concentrations (g/L, blue circle) in the medium were measured (upper panel). Relative diterpene contents (ferruginol equivalent mg/gDCW) and concentrations (ferruginol equivalent mg/L) profiles were measured for miltiradiene (black bar, square symbol) and abietatriene (red bar, circle symbol), respectively (lower panel). A fed-batch cultivation of the engineered strain was conducted, starting with 2% (w/v) initial glucose, followed by multiple glucose supplementations (1%, w/v) during the cultivation (right panel). Data represent mean values of at least triplicated cultivations, and error bars represent standard deviations. (B) A fed-batch cultivation of engineered strains (C. glutamicum HL01 harboring pBE-DI ptKtC) was conducted with truncation versions of KSL and CPS. LK stands for a linker. See the details above