Fig. 4

Analysis of the purification of FDCA product: (A) HPLC chromatograms of the final reaction solution obtained by G. oxydans DSM 50049 catalyzed oxidation of FFCA (1), FDCA recovered by extraction in ethyl acetate (2), FDCA remaining in the aqueous phase (3), and standard FDCA (4); (B) a picture of the purified FDCA, and (C) 1H-NMR of the purified FDCA, indicating > 99% purity