In vitro and preclinical evaluation of the antifungal activity of 6-methoxy-1 H-indole-2-carboxylic acid produced by Bacillus toyonensis strain OQ071612 formulated as nanosponge hydrogel

Table 1 Runs created by the Box-Behnken design and the observed responses

Run	A: Polymer-linker ratio	B: Homogenization speed	C: Homogenization time	D: PVA%	Particle size	PDI	EE
1	1:10	12,500	14	0.4	408	0.4	88
2	1:4	12,500	12	0.4	375	0.26	87
3	1:4	12,500	10	0.5	384	0.24	88
4	1:4	10,000	10	0.4	387	0.36	91
5	1:4	15,000	12	0.3	400	0.4	85.4
6	1:4	10,000	12	0.5	392	0.31	89.2
7	1:10	12,500	10	0.4	405	0.33	84
8	1:2	12,500	12	0.3	424	0.51	88
9	1:4	12,500	14	0.3	378	0.44	88.5
10	1:4	12,500	12	0.4	375	0.21	87.8
11	1:4	12,500	12	0.4	375	0.21	87.8
12	1:4	12,500	12	0.4	375	0.21	87.8
13	1:10	15,000	12	0.4	418	0.53	84.4
14	1:10	10,000	12	0.4	415	0.56	84.9
15	1:4	12,500	12	0.4	375	0.23	87.78
16	1:2	12,500	10	0.4	422	0.42	88.6
17	1:4	10,000	14	0.4	385	0.3	88.9
18	1:2	15,000	12	0.4	430	0.64	82
19	1:4	15,000	10	0.4	398	0.25	85
20	1:4	15,000	14	0.4	394	0.45	87
21	1:2	12,500	12	0.5	426	0.57	84
22	1:4	12,500	14	0.5	379	0.22	88.8
23	1:2	10,000	12	0.4	429	0.6	89
24	1:4	10,000	12	0.3	390	0.5	89.2
25	1:4	15,000	12	0.5	402	0.45	85.02
26	1:2	12,500	14	0.4	420	0.5	84.56
27	1:10	12,500	12	0.3	411	0.59	84.1
28	1:4	12,500	10	0.3	382	0.26	88.45
29	1:10	12,500	12	0.5	412	0.3	86.55

ISSN: 1475-2859