Fig. 4
From: Acetol biosynthesis enables NADPH balance during nitrogen limitation in engineered Escherichia coli
Central carbon metabolism and heterologous pathway for the production of acetol as represented in the metabolic network model. Metabolic flux distribution of acetol-producing engineered E. coli B4 during growth (A) and the nitrogen-limited phase (B) on 100% 2-13C glycerol as carbon and energy source. Metabolites: DHAP, dihydroxyacetone phosphate; GAP, glyceraldehyde 3-phosphate; FBP, fructose 1,6-bisphosphate; F6P fructose 6-phosphate; G1P, glucose-1-phosphate; G6P, glucose-6-phosphate; PG6, 6-phosphogluconate; R5P, ribose 5-phosphate; S7P, sedoheptulose 7-phosphate; E4P, erythrose 4-phosphate; KDPG, 2-keto-3-deoxy-6-phosphogluconate; BPG, 3-phosphoglyceric acid; SER, serine; PGA, 2-phosphoglyceric acid; PEP, phosphoenolpyruvate; PYR, pyruvate; Mal, malate; Fum, fumarate; Suc, succinate; OGA, 2-oxoglutaric acid; Icit, isocitrate; Act, aconitate; Cit, citrate; OAA, oxaloacetate; Glx, glyoxylate; AcCoA, acetyl-coA; CO2, carbondioxide. The numbers given represent fluxes normalized to the glycerol uptake, and the thickness and coloring of the arrows indicate the strength of the flux. Absolute and normalized fluxes are presented in Supplementary File S5. The expected labeling pattern is represented in [47]